Guardiola said: "They are human beings and they are disappointed because they gave it everything but we have to stand up".
City need one more win to bag the Premier League title, and would win it without kicking another ball should United lose on Sunday or at Bournemouth next weekend.
Spurs maintained their new-found momentum early in the second half, and now it was City who were struggling to regain the composure they had shown in the first 40 minutes of the match.
There are two games on Saturday evening.
City dropped just two points in its first 22 league matches this season, with that unbeaten sequence finally ended by a 4-3 defeat at Liverpool on January 14.
Chelsea kept their faint hopes of Champions League football next season alive by closing to within seven points of Spurs with a stunning eight-minute fightback to deepen Southampton's predicament in the bottom three. If they didn't win it, that would mean United winning all of their remaining six games, which they could easily do, and City losing at least five times. But in one week we have lost three games.
Ben Davies' horrific 27th-minute tackle on Kompany typified Spurs' frustrations but there was salvation before the break for the hosts as Aymeric Laporte's clearance fired into the City goal off Eriksen.
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It's no coincidence that Parakilas has been speaking out about the issue since the matter of user data on Facebook came to light. Zuckerberg responded saying: "Congressman, that is an accurate description of the prank website I made when I was a sophomore".
"We have a big chance here to create something for the future".
Unusually sluggish, Tottenham didn't test City and, with a swagger back in their stride, Guardiola's men took the lead in the 22nd minute.
City arrived at Wembley licking their wounds having been dumped out of the Champions League four days after Manchester United dramatically denied them the title. The top six really go at it with you.
Here's what Guardiola said when asked about the possibility of his team blowing their huge lead in the final weeks of the season.
Harry Kane will be expected to return to the starting line-up for Spurs after he marked his return to action from an ankle injury with a controversial goal.
Tottenham, meanwhile, will have to play without Danny Rose, after the left-back picked up a knock against Stoke.
Manchester City will have to do it without Fernandinho, who starts a two-game ban after accumulating 10 yellow cards.